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Optimizing Split Injections
03:09
Restek Corporation

Optimizing Split Injections

When performing a split injection, there are several considerations to keep in mind to improve the quality of your chromatography. But what should you change and why? In this Restek Tip, we’re going to show you how to optimize your split injections. We’ll start with liner selection, before discussing the importance of split ratios. Next, we’ll focus on optimizing the inlet temperature, and finally, the initial oven temperature. ----------------------------------------------------------------- ADDITIONAL RESOURCES GC Inlet Liner Selection: Split Liners, Part 1: https://www.restek.com/chromablography/chromablography/gc-inlet-liner-selection-part-ii-split-liners/ GC Inlet Liner Selection: Split Liners, Part 2: https://www.restek.com/chromablography/chromablography/gc-inlet-liner-selection-part-iib-split-liners-continued/ How to Choose a GC Inlet Liner: https://www.restek.com/technical-literature-library/articles/how-to-choose-a-GC-inlet-liner-simplify-selection-based-on-injection-type/ GC Inlet Maintenance Guide: https://www.restek.com/technical-literature-library/articles/GC-inlet-maintenance-resteks-quick-reference-guide/ ----------------------------------------------------------------- RESTEK RECOMMENDED TOOLS & PARTS Restek Inlet Liners: https://www.restek.com/liners/ Restek Electronic Leak Detector: https://www.restek.com/leakdetector/ ----------------------------------------------------------------- CONNECT WITH US: Comment on this video and subscribe to Restek's YouTube channel today at https://www.youtube.com/user/RestekCorp Website: https://www.restek.com Twitter: https://www.twitter.com/Restek Facebook: https://www.facebook.com/RestekCorporation LinkedIn: https://www.linkedin.com/company/restek-corporation/
3 Ways to Reduce Your Helium Usage
03:35
Restek Corporation

3 Ways to Reduce Your Helium Usage

Helium scarcity continues to be a pain point for gas chromatography. Prices are trending upward, supplies can be hard to source, and it’s a problem that isn’t going to go away. In this Restek Tip, we share three different ways to reduce your gas usage to get the most out of your helium cylinders. ----------------------------------------------------------------- Chapters 0:00 The Trouble with Helium 0:23 Leak Checking Your GC 1:05 GC Gas Saver Mode 2:22 Shutting Down Your GC 2:40 Alternate Carrier Gases ----------------------------------------------------------------- Additional Resources Benefits and Considerations of Converting to Hydrogen Carrier Gas | https://www.restek.com/technical-literature-library/articles/benefits-and-considerations-of-converting-to-hydrogen-carrier-gas/ Are You Experiencing Helium Supply Issues and Rising Costs? | https://www.restek.com/chromablography/chromablography/are-you-experiencing-helium-supply-issues-and-rising-costs/ Using Alternative Carrier Gases with Accelerated ASTM D2887 Simulated Distillation Analysis | https://www.restek.com/technical-literature-library/articles/using-alternative-carrier-gases-with-accelerated-ASTM-D2887-simulated-distillation-analysis/ ----------------------------------------------------------------- Restek Recommended Tools & Parts Restek Electronic Leak Detector | https://www.restek.com/products/accessories-labware/gc-accessories/gas-purification/8175/ Nitrogen & Hydrogen Gas Generators | https://www.restek.com/products/accessories-labware/gc-accessories/gas-generators/ ----------------------------------------------------------------- Connect with Us Comment on this video and subscribe to Restek's YouTube channel today at https://www.youtube.com/user/RestekCorp Website: https://www.restek.com LinkedIn: https://www.linkedin.com/company/restek-corporation/ Facebook: https://www.facebook.com/RestekCorporation Twitter: https://www.twitter.com/Restek
What You Need to Know About Gas Regulators
03:21
Restek Corporation

What You Need to Know About Gas Regulators

Gas regulators are fascinating devices and necessary tools in many chromatography applications. But with many options available and multiple variables to consider, choosing the right one can be a difficult task. In this Restek Tip, we share what you need to know about gas regulators. What are the differences between them? And what should you consider when selecting one? ----------------------------------------------------------------- Additional Resources How to connect 1/8 inch tubing to a Restek gas regulator | https://www.restek.com/chromablography/chromablography/how-to-connect-18-inch-tubing-to-a-restek-gas-regulator/ Need some help in choosing the correct gas regulator? | https://www.restek.com/chromablography/chromablography/need-some-help-in-choosing-the-correct-gas-regulator/ ----------------------------------------------------------------- Restek Recommended Tools & Parts Restek’s Ultra-High Purity Gas Regulators | https://www.restek.com/products/accessories-labware/gc-accessories/Ultra-High-Purity-Regulators/ Custom Ultra-High Purity Gas Regulator Quote Form | https://www.restek.com/forms/custom-ultra-high-purity-gas-regulator-quote-form/ ----------------------------------------------------------------- Connect with Us Comment on this video and subscribe to Restek's YouTube channel today at https://www.youtube.com/user/RestekCorp Website: https://www.restek.com LinkedIn: https://www.linkedin.com/company/restek-corporation/ Facebook: https://www.facebook.com/RestekCorporation Twitter: https://www.twitter.com/Restek
Installing the SPME Arrow Conversion Kit
02:34
Restek Corporation

Installing the SPME Arrow Conversion Kit

The SPME Arrow is a robust and versatile sample preparation device. To take full advantage of its many benefits, you first need to configure your GC. In this Restek Tip, we outline the parts you need, show you how to correctly install them, and answer some common questions. Solid phase microextraction, or SPME, is a fast and versatile sample preparation technique that reduces sample handling and solvent consumption. It can be used for headspace and immersion sampling while producing very clean extracts. To learn more about SPME, watch our ‘What is SPME?’ video here: https://youtu.be/YC201BnFGZc ----------------------------------------------------------------- Additional Resources How to Select the Correct Conversion Kit for Your SPME Arrow Fiber | https://www.restek.com/chromablography/chromablography/how-to-select-the-correct-conversion-kit-for-your-spme-arrow-fiber/ Set Your Sights on Superior Performance — Restek PAL SPME Arrow | https://www.restek.com/technical-literature-library/articles/set-your-sights-on-superior-performance/ ----------------------------------------------------------------- Restek Recommended Tools & Parts Restek PAL SPME Arrow GC Conversion Kits | https://www.restek.com/products/sample-preparation--air-sampling/sample-preparation-products/SPME/49963/ Restek PAL SPME Arrows | https://www.restek.com/products/sample-preparation--air-sampling/sample-preparation-products/SPME/49955/ ----------------------------------------------------------------- Connect with Us Comment on this video and subscribe to Restek’s YouTube channel today at https://www.youtube.com/user/RestekCorp Website: https://www.restek.com LinkedIn: https://www.linkedin.com/company/restek-corporation/ Facebook: https://www.facebook.com/RestekCorporation Twitter: https://www.twitter.com/Restek
What is SPME?
03:09
Restek Corporation

What is SPME?

Solid phase microextraction, or SPME, is a fast and versatile sample preparation technique that reduces sample handling and solvent consumption. It can be used for headspace and immersion sampling while producing very clean extracts. In this Restek Tip, we explore the basics of SPME. What is it? How does it work? And what are its advantages? ----------------------------------------------------------------- Additional Resources High-Performing Restek PAL SPME Fibers | https://www.restek.com/technical-literature-library/articles/high-performing-restek-pal-spme-fibers Set Your Sights on Superior Performance — Restek PAL SPME Arrow | https://www.restek.com/technical-literature-library/articles/set-your-sights-on-superior-performance/ ----------------------------------------------------------------- Restek Recommended Tools & Parts Restek PAL SPME Fibers | https://www.restek.com/products/sample-preparation--air-sampling/sample-preparation-products/SPME/47352/ Restek PAL SPME Arrows | https://www.restek.com/products/sample-preparation--air-sampling/sample-preparation-products/SPME/49955/ Restek PAL SPME Manual Injection Kit | https://www.restek.com/products/sample-preparation--air-sampling/sample-preparation-products/SPME/49957/ ----------------------------------------------------------------- Connect with Us Comment on this video and subscribe to Restek’s YouTube channel today at https://www.youtube.com/user/RestekCorp Website: https://www.restek.com LinkedIn: https://www.linkedin.com/company/restek-corporation/ Facebook: https://www.facebook.com/RestekCorporation Twitter: https://www.twitter.com/Restek
Optimizing Splitless Injections
03:40
Restek Corporation

Optimizing Splitless Injections

Splitless injections excel at trace analyses, but to get to get the most out of a splitless analysis, it’s important to optimize your system. But what should you optimize and what are the benefits? In this Restek Tip, we’re going to show you how to optimize your splitless injections. We’ll start with liner selection, before discussing the importance of the splitless hold time. Next, we’ll focus on optimizing the inlet temperature, and finally, the initial oven temperature. ----------------------------------------------------------------- ADDITIONAL RESOURCES GC Inlet Liner Selection: Splitless Liners: https://blog.restek.com/gc-inlet-liner-selection-part-i-splitless-liner-selection/ Calculating Splitless Hold Time: https://blog.restek.com/calculating-splitless-valve-time-for-splitless-injection-gc-with-the-new-ezgc-method-translator-and-flow-calculator-pesticides/ How to Choose a GC Inlet Liner: https://www.restek.com/pdfs/GNAR1991B-UNV.pdf GC Inlet Maintenance Guide: https://www.restek.com/technical-literature-library/articles/GC-inlet-maintenance-resteks-quick-reference-guide/ ----------------------------------------------------------------- RESTEK RECOMMENDED TOOLS & PARTS Restek Inlet Liners: https://www.restek.com/Supplies-Accessories/GC-Accessories/Inlet-Liners-Liner-Supplies Restek Electronic Leak Detector: https://www.restek.com/leakdetector ----------------------------------------------------------------- CONNECT WITH US: Comment on this video and subscribe to Restek's YouTube channel today at https://www.youtube.com/user/RestekCorp Website: https://www.restek.com Twitter: https://www.twitter.com/Restek Facebook: https://www.facebook.com/RestekCorporation LinkedIn: https://www.linkedin.com/company/restek-corporation/
Why Are There so Many C18 Columns?
03:05
Restek Corporation

Why Are There so Many C18 Columns?

From end-capped to aqueous, there are a multitude of C18 phases to choose from. But why are there so many? A C18 is a C18, right? In today’s Restek Tip, we’re going to explore the world of C18 phases. Are there benefits to choosing one specific to your analysis? And what parameters should you keep in mind when selecting a phase? ----------------------------------------------------------------- ADDITIONAL RESOURCES A C18 is a C18, right? | https://www.restek.com/chromablography/chromablography/a-c18-is-a-c18-right/ Why do I Lose Retention on my C18 in High Aqueous Mobile Phases? | https://www.restek.com/technical-literature-library/articles/tech-tip-why-do-i-lose-retention-on-my-C18-in-highly-aqueous-mobile-phases/ ----------------------------------------------------------------- RESTEK RECOMMENDED TOOLS & PARTS Raptor C18 LC Columns (traditional, end-capped columns) | https://www.restek.com/products/columns/HPLC--UHPLC-Columns/Analytical-Columns/43275/ Ultra Aqueous C18 LC Columns (100% aqueous phase columns) | https://www.restek.com/products/columns/HPLC--UHPLC-Columns/Analytical-Columns/2143/ Raptor ARC-18 LC Columns (sterically-protected, acid resistant columns) | https://www.restek.com/products/columns/HPLC--UHPLC-Columns/Analytical-Columns/41315/ ----------------------------------------------------------------- Connect with us: Comment on this video and subscribe to Restek's YouTube channel today at https://www.youtube.com/user/RestekCorp Website: https://www.restek.com Twitter: https://www.twitter.com/Restek Facebook: https://www.facebook.com/RestekCorporation LinkedIn: https://www.linkedin.com/company/restek-corporation/ GNAV3670-UNV
Rapid Screening & Confirmation of Analytes in Biofluids Using a Single Sample and Sampling Device
05:30
Restek Corporation

Rapid Screening & Confirmation of Analytes in Biofluids Using a Single Sample and Sampling Device

There is a need for mass spectrometry based analytical workflows that facilitate rapid screening and quantitation of target analytes in biological fluids. In an ideal world, the same sample can be used for both screening and confirmation. However, in most cases, two independent samples and sample-prep workflows are needed to isolate the target analytes for the screening, quantitation and confirmation steps. In this poster video, we present a technology that can be used to capture target compounds from diverse biofluid samples and perform rapid screening and quantitation via direct-to-MS experiments. In addition, the same device can be used for confirmation of said analytes via LC-MS/MS without further sample preparation. This novel technology, known as “coated blade spray” (CBS), provides reliable and reproducible results and with a total analysis time under 5 minutes for the rapid screening protocol. ----------------------------------------------------------------- Download a PDF of the full presentation: https://www.restek.com/globalassets/pdfs/presentations/poster-asms-2020-rapid-screening-and-confirmation-of-target-analytes-in-biological-fluids-using-a-single-sample-and-a-single-sampling-collection-device.pdf Want to know more about Restek coated blade spray (CBS) technology? Sign up today at https://www.restek.com/cbs ----------------------------------------------------------------- Connect with us: Comment on this video and subscribe to Restek's YouTube channel today at https://www.youtube.com/user/RestekCorp Website: https://www.restek.com Twitter: https://www.twitter.com/Restek Facebook: https://www.facebook.com/RestekCorporation LinkedIn: https://www.linkedin.com/company/restek-corporation/
LC-MS/MS Analysis of Glyphosate & Other Polar Contaminants in Food with an Ion Exchange/HILIC Column
04:19
Restek Corporation

LC-MS/MS Analysis of Glyphosate & Other Polar Contaminants in Food with an Ion Exchange/HILIC Column

Glyphosate is a broad-spectrum herbicide widely used throughout the world. The International Agency for Research on Cancer classified glyphosate as a probable human carcinogen. The safety residual level of glyphosate in food is regulated in the USA and Europe as well as other regions. Direct analysis of underivatized glyphosate, however, can be challenging due to its minimum retention on a reversed-phase or hydrophilic interaction chromatography (HILIC) column and severe adsorption onto the stainless-steel flow path. On the other hand, glyphosate and other polar anionic contaminants tend to strongly retain on an anion-exchange column and require high salt mobile phase to elute, which is not friendly to mass spectrometric analysis. These challenges motivated the development of a hybrid ion-exchange/HILIC column that offers balanced retention of glyphosate as well as other polar contaminants. With the column, an LC-MS/MS method has been established for the detection of glyphosate, aminomethylphosphonic acid (AMPA), glufosinate, and 14 other polar contaminants in various food matrices in a single run. Additionally, the nonspecific binding of glyphosate and other chelating compounds onto stainless steel is minimized with a simple passivation solution. ----------------------------------------------------------------- Download a PDF of the full presentation: https://www.restek.com/pdfs/ASMS_2020_Glyphosate_Food_PolarX.pdf ----------------------------------------------------------------- Connect with us: Comment on this video and subscribe to Restek's YouTube channel today at https://www.youtube.com/user/RestekCorp Website: https://www.restek.com Twitter: https://www.twitter.com/Restek Facebook: https://www.facebook.com/RestekCorporation LinkedIn: https://www.linkedin.com/company/restek-corporation/
 

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